› 7vui

Electron Microscopy
3.3Å resolution

Cryo-EM structure of a class A orphan GPCR

Released:
DOI: 10.2210/pdb7vui/pdb
PDB entry 7vui coloured by chain, front view.
PDB entry 7vui coloured by chain, side view.
PDB entry 7vui coloured by chain, top view.
Source organisms:
Primary publication:
Molecular insights into ligand recognition and G protein coupling of the neuromodulatory orphan receptor GPR139.
Zhou Y, Daver H, Trapkov B, Wu L, Wu M, Harpsøe K, Gentry PR, Liu K, Larionova M, Liu J, Chen N, Bräuner-Osborne H, Gloriam DE, Hua T, Liu ZJ
OpenAccess logo Cell Res 32 210-213 (2022)
PMID: 34916631
Related structures: EMD-32129

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-209422 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 30.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63092 (Residues: 5-64, 204-371; Coverage: 55%)
  • Best match: P63092-2 (Residues: 5-64, 190-357)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 346 amino acids
Theoretical weight: 38.05 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 157 amino acids
Theoretical weight: 17.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Endo-1,4-beta-xylanase; Probable G-protein coupled receptor 139 Chain: R
Molecule details ›
Chain: R
Length: 533 amino acids
Theoretical weight: 60.89 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P09850 (Residues: 29-213; Coverage: 100%)
  • Canonical: Q6DWJ6 (Residues: 2-321; Coverage: 91%)
Gene names: GPR139, GPRG1, PGR3, xlnA
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 1 x MG
Ligand 7ZQ 1 x 7ZQ
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-32129
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda

Quick links

Citations

1 review citation

New insights into GPCR coupling and dimerisation from cryo-EM structures.
Gusach et al. (2023)
7 other articles

1 mention without citation

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)