› 7vuz

Electron Microscopy
2.89Å resolution

Cryo-EM structure of pseudoallergen receptor MRGPRX2 complex with PAMP-12, state2

Released:
DOI: 10.2210/pdb7vuz/pdb
PDB entry 7vuz coloured by chain, front view.
PDB entry 7vuz coloured by chain, side view.
PDB entry 7vuz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure, function and pharmacology of human itch receptor complexes.
Yang F, Guo L, Li Y, Wang G, Wang J, Zhang C, Fang GX, Chen X, Liu L, Yan X, Liu Q, Qu C, Xu Y, Xiao P, Zhu Z, Li Z, Zhou J, Yu X, Gao N, Sun JP
Nature 600 164-169 (2021)
PMID: 34789875
Related structures: EMD-32132

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-225724 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Mas-related G-protein coupled receptor member X2 Chain: R
Molecule details ›
Chain: R
Length: 330 amino acids
Theoretical weight: 37.12 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q96LB1 (Residues: 1-330; Coverage: 100%)
Gene names: MRGPRX2, MRGX2
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 358 amino acids
Theoretical weight: 39.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 58 amino acids
Theoretical weight: 6.38 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 5-62; Coverage: 82%)
Gene name: GNG2
Sequence domains: GGL domain
scFv Chain: S
Molecule details ›
Chain: S
Length: 285 amino acids
Theoretical weight: 30.36 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda

Ligands and Environments

1 bound ligand:
Ligand CLR 1 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.89Å
Relevant EMDB volumes: EMD-32132
Expression system: Spodoptera frugiperda

Quick links

Citations

9 review citations

Specialized Pro-Resolving Mediators as Resolution Pharmacology for the Control of Pain and Itch.
Ji RR. (2023)
8 more

1 mention without citation

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)