› 7vvn

Electron Microscopy
3.8Å resolution

PTH-bound human PTH1R in complex with Gs (class4)

Released:
DOI: 10.2210/pdb7vvn/pdb
PDB entry 7vvn coloured by chain, front view.
PDB entry 7vvn coloured by chain, side view.
PDB entry 7vvn coloured by chain, top view.
Source organisms:
Primary publication:
Endogenous ligand recognition and structural transition of a human PTH receptor.
Kobayashi K, Kawakami K, Kusakizako T, Miyauchi H, Tomita A, Kobayashi K, Shihoya W, Yamashita K, Nishizawa T, Kato HE, Inoue A, Nureki O
Mol Cell 82 3468-3483.e5 (2022)
PMID: 35932760
Related structures: EMD-32145

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-224946 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 44.26 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P63092 (Residues: 5-394; Coverage: 96%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 351 amino acids
Theoretical weight: 38.74 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 68 amino acids
Theoretical weight: 7.55 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 1-67; Coverage: 94%)
Gene name: GNG2
Sequence domains: GGL domain
nanobody Nb35 Chain: N
Molecule details ›
Chain: N
Length: 137 amino acids
Theoretical weight: 15.02 KDa
Source organism: unidentified
Expression system: Escherichia coli
Parathyroid hormone Chain: P
Molecule details ›
Chain: P
Length: 34 amino acids
Theoretical weight: 4.13 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01270 (Residues: 32-65; Coverage: 38%)
Gene name: PTH
Sequence domains: Parathyroid hormone family
Parathyroid hormone/parathyroid hormone-related peptide receptor Chain: R
Molecule details ›
Chain: R
Length: 473 amino acids
Theoretical weight: 54.26 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q03431 (Residues: 27-491; Coverage: 82%)
Gene names: PTH1R, PTHR, PTHR1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-32145
Expression systems:
  • Homo sapiens
  • Spodoptera frugiperda
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Parathyroid Hormone-Related Protein/Parathyroid Hormone Receptor 1 Signaling in Cancer and Metastasis.
Zhao et al. (2023)
10 other articles

1 mention without citation

New Insights into the Structure and Function of Class B1 GPCRs.
Cary et al. (2023)