› 7vxw

X-ray diffraction
2.22Å resolution

Snapshots of Human PSMD10(Gankyrin) unfolding by urea: 1 hour incubation

Released:
DOI: 10.2210/pdb7vxw/pdb
PDB entry 7vxw coloured by chain, front view.
PDB entry 7vxw coloured by chain, side view.
PDB entry 7vxw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Snapshots of urea-induced early structural changes and unfolding of an ankyrin repeat protein at atomic resolution.
Medur Gurushankar MS, Dalvi S, Venkatraman P
Protein Sci 31 e4515 (2022)
PMID: 36382986

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131139 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
26S proteasome non-ATPase regulatory subunit 10 Chain: A
Molecule details ›
Chain: A
Length: 226 amino acids
Theoretical weight: 24.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75832 (Residues: 1-226; Coverage: 100%)
Gene name: PSMD10
Sequence domains: Ankyrin repeats (3 copies)

Ligands and Environments

1 bound ligand:
Ligand URE 3 x URE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P3221
Unit cell:
a: 60.19Å b: 60.19Å c: 122.29Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.25 0.242 0.327
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Snapshots of urea-induced early structural changes and unfolding of an ankyrin repeat protein at atomic resolution.
Medur Gurushankar et al. (2022)