› 7vyo

X-ray diffraction
2.25Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183038 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GdmN Chains: A, B
Molecule details ›
Chains: A, B
Length: 682 amino acids
Theoretical weight: 74.37 KDa
Source organism: Streptomyces hygroscopicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q84G19 (Residues: 1-682; Coverage: 100%)
Gene name: gdmN
Sequence domains:

Ligands and Environments

7 bound ligands:
Ligand SO4 10 x SO4
Ligand FE 2 x FE
Ligand GOL 1 x GOL
Ligand EDO 10 x EDO
Ligand PEG 2 x PEG
Ligand CP 1 x CP
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P3121
Unit cell:
a: 110.955Å b: 110.955Å c: 231.544Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.176 0.209
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Endowing homodimeric carbamoyltransferase GdmN with iterative functions through structural characterization and mechanistic studies.
Wei et al. (2022)