› 7w15

X-ray diffraction
1.77Å resolution

Crystal Structure of MPH-E in complex with GTP and Erythromycin

Released:
DOI: 10.2210/pdb7w15/pdb
PDB entry 7w15 coloured by chain, front view.
PDB entry 7w15 coloured by chain, side view.
PDB entry 7w15 coloured by chain, top view.
Source organism: Acinetobacter baumannii
Entry authors: Qi Q, Kuang L, Jiang Y

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107780 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside phosphotransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 302 amino acids
Theoretical weight: 34.62 KDa
Source organism: Acinetobacter baumannii
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A5Y459 (Residues: 1-294; Coverage: 100%)
Gene names: A6Y97_19020, B9W25_15335, CBE85_17540, CV955_0020335, F4T85_17960, FJU36_20235, FR761_18675, GNY86_18375, GSE42_20205, H0529_21860, IAG11_16870, J6E47_19550, P9867_16905, SAMEA104305318_04078, SAMEA104305340_03884, SAMEA4394745_03588, mph(E), mph2, pNDM-AB_013
Sequence domains: Phosphotransferase enzyme family

Ligands and Environments

4 bound ligands:
Ligand ERY 2 x ERY
Ligand GTP 2 x GTP
Ligand MG 6 x MG
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P21
Unit cell:
a: 84.62Å b: 50.278Å c: 97.396Å
α: 90° β: 115.41° γ: 90°
R-values:
R R work R free
0.188 0.188 0.196
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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