› 7w2z

Electron Microscopy
2.8Å resolution

Cryo-EM structure of the ghrelin-bound human ghrelin receptor-Go complex

Released:
DOI: 10.2210/pdb7w2z/pdb
PDB entry 7w2z coloured by chain, front view.
PDB entry 7w2z coloured by chain, side view.
PDB entry 7w2z coloured by chain, top view.
Source organisms:
Primary publication:
Molecular mechanism of agonism and inverse agonism in ghrelin receptor.
Qin J, Cai Y, Xu Z, Ming Q, Ji SY, Wu C, Zhang H, Mao C, Shen DD, Hirata K, Ma Y, Yan W, Zhang Y, Shao Z
OpenAccess logo Nat Commun 13 300 (2022)
PMID: 35027551
Related structures: EMD-32268

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-209412 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(o) subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.46 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P09471 (Residues: 4-56, 182-354; Coverage: 64%)
Gene name: GNAO1
Sequence domains: G-protein alpha subunit
Growth hormone secretagogue receptor type 1 Chain: R
Molecule details ›
Chain: R
Length: 366 amino acids
Theoretical weight: 41.36 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q92847 (Residues: 1-366; Coverage: 100%)
Gene name: GHSR
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
ScFv16 Chain: S
Molecule details ›
Chain: S
Length: 250 amino acids
Theoretical weight: 26.61 KDa
Source organism: synthetic construct
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 339 amino acids
Theoretical weight: 37.29 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Ghrelin-27 Chain: L
Molecule details ›
Chain: L
Length: 16 amino acids
Theoretical weight: 2 KDa
Source organism: Homo sapiens
Expression system: synthetic construct
UniProt:
  • Canonical: Q9UBU3 (Residues: 24-39; Coverage: 17%)
Gene names: GHRL, MTLRP, UNQ524/PRO1066
Sequence domains: Motilin/ghrelin

Ligands and Environments

1 bound ligand:
Ligand CLR 2 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-32268
Expression systems:
  • Spodoptera frugiperda
  • synthetic construct

Quick links

Citations

4 review citations

New insights into GPCR coupling and dimerisation from cryo-EM structures.
Gusach et al. (2023)
3 more

1 mention without citation

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)