› 7w4s

X-ray diffraction
2.6Å resolution

Structure of the M. tuberculosis HtrA S363A mutant at room-temperature

Released:
DOI: 10.2210/pdb7w4s/pdb
PDB entry 7w4s coloured by chain, front view.
PDB entry 7w4s coloured by chain, side view.
PDB entry 7w4s coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Allosteric Determinants in High Temperature Requirement A Enzymes Are Conserved and Regulate the Population of Active Conformations.
Gupta AK, Singh K, Patidar Y, Sharma R, Sardesai AA, Reddy G, Gopal B
ACS Chem Biol (2023)
PMID: 37319329

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126511 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable serine protease HtrA1 Chain: A
Molecule details ›
Chain: A
Length: 314 amino acids
Theoretical weight: 32.51 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O06291 (Residues: 223-528; Coverage: 58%)
Gene names: Rv1223, degP, htrA, htrA1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: R3
Unit cell:
a: 109Å b: 109Å c: 63.02Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.191 0.229
Expression system: Escherichia coli BL21(DE3)

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