› 7w6q

X-ray diffraction
2.2Å resolution

Crystal structure of a PSH1 in complex with ligand J1K

Released:
DOI: 10.2210/pdb7w6q/pdb
PDB entry 7w6q coloured by chain, front view.
PDB entry 7w6q coloured by chain, side view.
PDB entry 7w6q coloured by chain, top view.
Source organism: unidentified
Primary publication:
Multiple Substrate Binding Mode-Guided Engineering of a Thermophilic PET Hydrolase.
Pfaff L, Gao J, Li Z, Jäckering A, Weber G, Mican J, Chen Y, Dong W, Han X, Feiler CG, Ao YF, Badenhorst CPS, Bednar D, Palm GJ, Lammers M, Damborsky J, Strodel B, Liu W, Bornscheuer UT, Wei R
OpenAccess logo ACS Catal 12 9790-9800 (2022)
PMID: 35966606

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169040 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PSH1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 258 amino acids
Theoretical weight: 27.85 KDa
Source organism: unidentified
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand J1K 1 x J1K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17B1
Spacegroup: P21
Unit cell:
a: 52.705Å b: 56.219Å c: 100.628Å
α: 90° β: 93.79° γ: 90°
R-values:
R R work R free
0.227 0.225 0.26
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Microbial Enzyme Biotechnology to Reach Plastic Waste Circularity: Current Status, Problems and Perspectives.
Orlando et al. (2023)
10 more

2 mentions without citation

Recent advances in the biodegradation of polyethylene terephthalate with cutinase-like enzymes.
Sui et al. (2023)
1 more