› 7w6y

X-ray diffraction
3.1Å resolution

Crystal structure of Kangiella koreensis RseP orthologue in complex with batimastat in space group P1

Released:
DOI: 10.2210/pdb7w6y/pdb
PDB entry 7w6y coloured by chain, front view.
PDB entry 7w6y coloured by chain, side view.
PDB entry 7w6y coloured by chain, top view.
Source organism: Kangiella koreensis DSM 16069
Primary publication:
Mechanistic insights into intramembrane proteolysis by E. coli site-2 protease homolog RseP.
Imaizumi Y, Takanuki K, Miyake T, Takemoto M, Hirata K, Hirose M, Oi R, Kobayashi T, Miyoshi K, Aruga R, Yokoyama T, Katagiri S, Matsuura H, Iwasaki K, Kato T, Kaneko MK, Kato Y, Tajiri M, Akashi S, Nureki O, Hizukuri Y, Akiyama Y, Nogi T
OpenAccess logo Sci Adv 8 eabp9011 (2022)
PMID: 36001659

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111752 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Zinc metalloprotease Chain: A
Molecule details ›
Chain: A
Length: 456 amino acids
Theoretical weight: 50.44 KDa
Source organism: Kangiella koreensis DSM 16069
Expression system: Escherichia coli
UniProt:
  • Canonical: C7R5Z1 (Residues: 1-445; Coverage: 100%)
Gene name: Kkor_1905
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand BAT 1 x BAT
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P1
Unit cell:
a: 44.56Å b: 49.78Å c: 76.08Å
α: 86.897° β: 79.181° γ: 82.189°
R-values:
R R work R free
0.258 0.256 0.299
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The role of site-2-proteases in bacteria: a review on physiology, virulence, and therapeutic potential.
Kristensen et al. (2023)
5 other articles

1 mention without citation

Mechanistic insights into intramembrane proteolysis by E. coli site-2 protease homolog RseP.
Imaizumi et al. (2022)