› 7w82

X-ray diffraction
3.1Å resolution

Crystal structure of maize RDR2

Released:
DOI: 10.2210/pdb7w82/pdb
PDB entry 7w82 coloured by chain, front view.
PDB entry 7w82 coloured by chain, side view.
PDB entry 7w82 coloured by chain, top view.
Source organism: Zea mays
Primary publication:
Structure of plant RNA-DEPENDENT RNA POLYMERASE 2, an enzyme involved in small interfering RNA production.
Du X, Yang Z, Ariza AJF, Wang Q, Xie G, Li S, Du J
Plant Cell 34 2140-2149 (2022)
PMID: 35188193

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172764 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-dependent RNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 1024 amino acids
Theoretical weight: 115.39 KDa
Source organism: Zea mays
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q19VG2 (Residues: 107-1127; Coverage: 91%)
Gene names: 100502460, ZEAMMB73_Zm00001d003378, mop1
Sequence domains: RNA dependent RNA polymerase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: R32
Unit cell:
a: 149.036Å b: 149.036Å c: 345.473Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.23 0.27
Expression system: Spodoptera frugiperda

Quick links

Citations

5 citation in other articles

Enzymatic reactions of AGO4 in RNA-directed DNA methylation: siRNA duplex loading, passenger strand elimination, target RNA slicing, and sliced target retention.
Wang et al. (2023)
4 more

1 mention without citation

Predicted models and CCP4.
Simpkin et al. (2023)