› 7w8y

X-ray diffraction
1.39Å resolution

DMSPP- and Naplha-Me-Trp-bound 6-dimethylallyl tryptophan synthase, IptA

Released:
DOI: 10.2210/pdb7w8y/pdb
PDB entry 7w8y coloured by chain, front view.
PDB entry 7w8y coloured by chain, side view.
PDB entry 7w8y coloured by chain, top view.
Source organism: Streptomyces sp. SN-593
Primary publication:
Crystal structures of a 6-dimethylallyltryptophan synthase, IptA: Insights into substrate tolerance and enhancement of prenyltransferase activity.
Suemune H, Nishimura D, Mizutani K, Sato Y, Hino T, Takagi H, Shiozaki-Sato Y, Takahashi S, Nagano S
Biochem Biophys Res Commun 593 144-150 (2022)
PMID: 35074664

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112810 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan 6-prenyltransferase Chain: A
Molecule details ›
Chain: A
Length: 369 amino acids
Theoretical weight: 39.73 KDa
Source organism: Streptomyces sp. SN-593
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D6RT90 (Residues: 20-385; Coverage: 95%)
Gene names: RVR_4274, iptA
Sequence domains: Tryptophan dimethylallyltransferase

Ligands and Environments

5 bound ligands:
Ligand DST 1 x DST
Ligand SO4 1 x SO4
Ligand TRS 1 x TRS
Ligand DTT 1 x DTT
Ligand E9M 1 x E9M
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P21
Unit cell:
a: 49.521Å b: 44.714Å c: 78.384Å
α: 90° β: 97.325° γ: 90°
R-values:
R R work R free
0.214 0.212 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Open-Source Browser-Based Tools for Structure-Based Computer-Aided Drug Discovery.
Wang et al. (2022)
1 other article