› 7wa4

X-ray diffraction
3.5Å resolution

Crystal structure of GIGANTEA in complex with LKP2

Released:
DOI: 10.2210/pdb7wa4/pdb
PDB entry 7wa4 coloured by chain, front view.
PDB entry 7wa4 coloured by chain, side view.
PDB entry 7wa4 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural analysis of the regulation of blue-light receptors by GIGANTEA.
Kwon E, Pathak D, Dahal P, Tandukar S, Jung HS, Kim WY, Kim DY
Cell Rep 39 110700 (2022)
PMID: 35443175

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186658 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein GIGANTEA Chain: A
Molecule details ›
Chain: A
Length: 815 amino acids
Theoretical weight: 89.44 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli B
UniProt:
  • Canonical: Q9SQI2 (Residues: 1-813; Coverage: 69%)
Gene names: At1g22770, GI, T22J18.6
Adagio protein 2 Chain: B
Molecule details ›
Chain: B
Length: 138 amino acids
Theoretical weight: 15.32 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli B
UniProt:
  • Canonical: Q8W420 (Residues: 29-164; Coverage: 22%)
Gene names: ADO2, At2g18915, F19F24.11, FKL1, LKP2
Sequence domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand FMN 1 x FMN
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 11C
Spacegroup: P61
Unit cell:
a: 104.75Å b: 104.75Å c: 232.161Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.25 0.245 0.294
Expression system: Escherichia coli B

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