› 7waj

X-ray diffraction
2.25Å resolution

Glutamyl-tRNA synthetase from Plasmodium falciparum (PfERS) complexed with ATP and Co

Released:
DOI: 10.2210/pdb7waj/pdb
PDB entry 7waj coloured by chain, front view.
PDB entry 7waj coloured by chain, side view.
PDB entry 7waj coloured by chain, top view.
Source organism: Plasmodium falciparum 3D7
Primary publication:
Structural characterization of glutamyl-tRNA synthetase (GluRS) from Plasmodium falciparum.
Sharma VK, Chhibber-Goel J, Yogavel M, Sharma A
Mol Biochem Parasitol 253 111530 (2022)
PMID: 36370911

Function and Biology Details

Reaction catalysed: 
ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184745 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
glutamate--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 523 amino acids
Theoretical weight: 61.33 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8IDK7 (Residues: 305-823; Coverage: 60%)
Gene name: PF3D7_1349200
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand BME 1 x BME
Ligand ATP 1 x ATP
Ligand CO 8 x CO
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: C2
Unit cell:
a: 158.19Å b: 46.34Å c: 95.32Å
α: 90° β: 110.72° γ: 90°
R-values:
R R work R free
0.201 0.199 0.247
Expression system: Escherichia coli BL21(DE3)

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