› 7wgu

X-ray diffraction
1.85Å resolution

Crystal structure of metal-binding protein EfeO from Escherichia coli

Released:
DOI: 10.2210/pdb7wgu/pdb
PDB entry 7wgu coloured by chain, front view.
PDB entry 7wgu coloured by chain, side view.
PDB entry 7wgu coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of EfeB and EfeO in a bacterial siderophore-independent iron transport system
Nakatsuji S, Okumura K, Takase R, Watanabe D, Mikami B, Hashimoto W
Biochem. Biophys. Res. Commun. 594 124-130 (2022)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-221685 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron uptake system component EfeO Chains: A, B
Molecule details ›
Chains: A, B
Length: 358 amino acids
Theoretical weight: 39.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AB24 (Residues: 27-375; Coverage: 100%)
Gene names: JW1003, b1018, efeO, ycdO
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand EDO 30 x EDO
Ligand ACT 1 x ACT
Ligand PGE 1 x PGE
Ligand SO4 1 x SO4
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: C2
Unit cell:
a: 139.907Å b: 51.877Å c: 117.379Å
α: 90° β: 112.44° γ: 90°
R-values:
R R work R free
0.202 0.199 0.244
Expression system: Escherichia coli

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