› 7wig

Electron Microscopy
2.7Å resolution

Cryo-EM structure of the L-054,264-bound human SSTR2-Gi1 complex

Released:
DOI: 10.2210/pdb7wig/pdb
PDB entry 7wig coloured by chain, front view.
PDB entry 7wig coloured by chain, side view.
PDB entry 7wig coloured by chain, top view.
Source organisms:
Primary publication:
Structures of the endogenous peptide- and selective non-peptide agonist-bound SSTR2 signaling complexes.
Chen LN, Wang WW, Dong YJ, Shen DD, Guo J, Yu X, Qin J, Ji SY, Zhang H, Shen Q, He Q, Yang B, Zhang Y, Li Q, Mao C
Cell Res (2022)
PMID: 35578016
Related structures: EMD-32529

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-225581 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Somatostatin receptor type 2 Chain: R
Molecule details ›
Chain: R
Length: 369 amino acids
Theoretical weight: 41.37 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P30874 (Residues: 1-369; Coverage: 100%)
Gene name: SSTR2
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.41 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 345 amino acids
Theoretical weight: 37.92 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 70 amino acids
Theoretical weight: 7.73 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63212 (Residues: 2-71; Coverage: 99%)
Gene name: GNG2
Sequence domains: GGL domain
single Fab chain (svFv16) Chain: S
Molecule details ›
Chain: S
Length: 269 amino acids
Theoretical weight: 28.81 KDa
Source organism: synthetic construct
Expression system: Trichoplusia ni

Ligands and Environments

1 bound ligand:
Ligand 9FI 1 x 9FI
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-32529
Expression systems:
  • Spodoptera frugiperda
  • Trichoplusia ni

Quick links

Citations

8 citation in other articles

Molecular basis for the selective G protein signaling of somatostatin receptors.
Chen et al. (2023)
7 more

1 mention without citation

Identification of a Novel SSTR3 Full Agonist for the Treatment of Nonfunctioning Pituitary Adenomas.
Modena et al. (2023)