› 7wj4

Electron Microscopy
3.15Å resolution

Structural basis for ligand binding modes of CTP synthase

Released:
DOI: 10.2210/pdb7wj4/pdb
PDB entry 7wj4 coloured by chain, front view.
PDB entry 7wj4 coloured by chain, side view.
PDB entry 7wj4 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structural basis for ligand binding modes of CTP synthase.
Zhou X, Guo CJ, Chang CC, Zhong J, Hu HH, Lu GM, Liu JL
OpenAccess logo Proc Natl Acad Sci U S A 118 (2021)
PMID: 34301892
Related structures: EMD-32542

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194307 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CTP synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 556 amino acids
Theoretical weight: 62.44 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q9VUL1 (Residues: 1-556; Coverage: 89%)
Gene names: CG45070, CTPsyn, Ctps
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand GTP 4 x GTP
Ligand ATP 4 x ATP
Ligand GGL 4 x GGL
Ligand MG 12 x MG
Ligand UTP 4 x UTP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.15Å
Relevant EMDB volumes: EMD-32542
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

3 review citations

Structural insights into the half-of-sites reactivity in homodimeric and homotetrameric metalloenzymes.
Nguyen et al. (2023)
2 more