› 7wj5

Electron Microscopy
3.72Å resolution

Cryo-EM structure of human somatostatin receptor 2 complex with its agonist somatostatin delineates the ligand binding specificity

Released:
DOI: 10.2210/pdb7wj5/pdb
PDB entry 7wj5 coloured by chain, front view.
PDB entry 7wj5 coloured by chain, side view.
PDB entry 7wj5 coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structure of the human somatostatin receptor 2 complex with its agonist somatostatin delineates the ligand-binding specificity.
Heo Y, Yoon E, Jeon YE, Yun JH, Ishimoto N, Woo H, Park SY, Song JJ, Lee W
OpenAccess logo Elife 11 (2022)
PMID: 35446253
Related structures: EMD-32543

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-209531 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 358 amino acids
Theoretical weight: 39.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Gai1 antibody (scfv16) Chain: E
Molecule details ›
Chain: E
Length: 266 amino acids
Theoretical weight: 28.64 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Soluble cytochrome b562; Somatostatin receptor type 2 Chain: R
Molecule details ›
Chain: R
Length: 518 amino acids
Theoretical weight: 58.16 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0ABE7 (Residues: 23-127; Coverage: 99%)
  • Canonical: P30874 (Residues: 1-369; Coverage: 100%)
Gene names: SSTR2, cybC
Sequence domains:
Somatostatin-14 Chain: S
Molecule details ›
Chain: S
Length: 14 amino acids
Theoretical weight: 1.64 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P61278 (Residues: 103-116; Coverage: 15%)
Gene name: SST
Sequence domains: Somatostatin/Cortistatin family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.72Å
Relevant EMDB volumes: EMD-32543
Expression system: Spodoptera frugiperda

Quick links

Citations

8 citation in other articles

Cryo-EM structure of the human somatostatin receptor 2 complex with its agonist somatostatin delineates the ligand-binding specificity.
Heo et al. (2022)
7 more

1 mention without citation

Identification of a Novel SSTR3 Full Agonist for the Treatment of Nonfunctioning Pituitary Adenomas.
Modena et al. (2023)