7wju

Electron Microscopy
2.69Å resolution

Cryo-EM structure of the AsCas12f1-sgRNAv1-dsDNA ternary complex

Released:
Entry authors: Wu Z, Liu D, Shen H, Ji Q
Related structures: EMD-32548

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-104531 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated endodeoxyribonuclease Cas12f1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 422 amino acids
Theoretical weight: 48.72 KDa
Source organism: Acidibacillus sulfuroxidans
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2U3D0N8 (Residues: 1-422; Coverage: 100%)
Gene names: BM613_13600, cas12f1
Sequence domains: Putative transposase DNA-binding domain
Target strand Chain: D
Molecule details ›
Chain: D
Length: 30 nucleotides
Theoretical weight: 9.26 KDa
Source organism: synthetic construct
Expression system: synthetic construct
Non-target strand Chain: E
sgRNAv1 Chain: C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.69Å
Relevant EMDB volumes: EMD-32548
Expression systems:
  • Escherichia coli
  • synthetic construct