› 7wkr

X-ray diffraction
1.6Å resolution

Room temperature structure of lysozyme solved by serial synchrotron crystallography

Released:
DOI: 10.2210/pdb7wkr/pdb
PDB entry 7wkr coloured by chain, front view.
PDB entry 7wkr coloured by chain, side view.
PDB entry 7wkr coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Combination of an inject-and-transfer system for serial femtosecond crystallography.
Lee K, Kim J, Baek S, Park J, Park S, Lee JL, Chung WK, Cho Y, Nam KH
J Appl Crystallogr 55 813-822 (2022)
PMID: 35979068

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
Expression system: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 11C
Spacegroup: P43212
Unit cell:
a: 78.53Å b: 78.53Å c: 38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.198
Expression system: Gallus gallus

Quick links

Citations

3 citation in other articles

Data of pink-beam serial synchrotron crystallography at the Pohang Light Source II.
Kim et al. (2024)
2 more

1 mention without citation

Combination of an inject-and-transfer system for serial femtosecond crystallography.
Lee et al. (2022)