› 7wlw

X-ray diffraction
2.9Å resolution

X-ray structure of thermostabilized Drosophila dopamine transporter with GABA transporter1-like substitutions in the binding site, in complex with SKF89976a

Released:
DOI: 10.2210/pdb7wlw/pdb
PDB entry 7wlw coloured by chain, front view.
PDB entry 7wlw coloured by chain, side view.
PDB entry 7wlw coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into GABA transport inhibition using an engineered neurotransmitter transporter.
Joseph D, Nayak SR, Penmatsa A
EMBO J e110735 (2022)
PMID: 35796008

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-226296 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Sodium-dependent dopamine transporter Chain: A
Molecule details ›
Chain: A
Length: 536 amino acids
Theoretical weight: 60.14 KDa
Source organism: Drosophila melanogaster
Expression system: Homo sapiens
UniProt:
  • Canonical: Q7K4Y6 (Residues: 25-601; Coverage: 85%)
Gene names: CG8380, DAT, fmn
Sequence domains: Sodium:neurotransmitter symporter family
Antibody fragment light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.31 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
Antibody Fragment heavy chain Chain: H
Molecule details ›
Chain: H
Length: 219 amino acids
Theoretical weight: 23.62 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda

Ligands and Environments

6 bound ligands:
Ligand CL 1 x CL
Ligand NA 2 x NA
Ligand 1WR 2 x 1WR
Ligand Y01 1 x Y01
Ligand DMU 1 x DMU
Ligand CLR 1 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 11.2C
Spacegroup: P212121
Unit cell:
a: 96.78Å b: 141.755Å c: 168.236Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.235 0.264
Expression systems:
  • Homo sapiens
  • Spodoptera frugiperda

Quick links

Citations

1 review citation

A comparative review on the well-studied GAT1 and the understudied BGT-1 in the brain.
Bhatt et al. (2023)
3 other articles

1 mention without citation

Analysis of Different Binding Modes for Tiagabine within the GAT-1 Transporter.
Łątka et al. (2022)