› 7wme

X-ray diffraction
1.7Å resolution

Crystal Structure of the catalytic domain of At-HIGLE

Released:
DOI: 10.2210/pdb7wme/pdb
PDB entry 7wme coloured by chain, front view.
PDB entry 7wme coloured by chain, side view.
PDB entry 7wme coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Holliday junction resolution by At-HIGLE: an SLX1 lineage endonuclease from Arabidopsis thaliana with a novel in-built regulatory mechanism.
Verma P, Kumari P, Negi S, Yadav G, Gaur V
OpenAccess logo Nucleic Acids Res 50 4630-4646 (2022)
PMID: 35412622

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179316 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Structure-specific endonuclease subunit SLX1 homolog Chain: A
Molecule details ›
Chain: A
Length: 183 amino acids
Theoretical weight: 20.78 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q682H4 (Residues: 1-183; Coverage: 50%)
Gene names: At2g30350, HIGLE, T9D9.16, T9D9_16
Sequence domains: GIY-YIG catalytic domain

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 40.518Å b: 53.452Å c: 69.947Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.164 0.194
Expression system: Escherichia coli

Quick links

Citations

1 review citation

DNA Holliday Junction: History, Regulation and Bioactivity.
Song et al. (2022)
2 other articles

1 mention without citation

Holliday junction resolution by At-HIGLE: an SLX1 lineage endonuclease from Arabidopsis thaliana with a novel in-built regulatory mechanism.
Verma et al. (2022)