› 7wnp

Hybrid

Crystallographic structure of copper amine oxidase from Arthrobacter glibiformis at pD 7.4 determined by both X-ray and neutron diffraction data at 1.72 angstrom resolution.

Released:
DOI: 10.2210/pdb7wnp/pdb
PDB entry 7wnp coloured by chain, front view.
PDB entry 7wnp coloured by chain, side view.
PDB entry 7wnp coloured by chain, top view.
Source organism: Arthrobacter globiformis
Primary publication:
Re-evaluation of protein neutron crystallography with and without X-ray/neutron joint refinement.
Murakawa T, Kurihara K, Adachi M, Kusaka K, Tanizawa K, Okajima T
OpenAccess logo IUCrJ 9 342-348 (2022)
PMID: 35546796

Function and Biology Details

Reaction catalysed: 
RCH(2)NH(2) + H(2)O + O(2) = RCHO + NH(3) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155528 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylethylamine oxidase Chain: X
Molecule details ›
Chain: X
Length: 621 amino acids
Theoretical weight: 68.99 KDa
Source organism: Arthrobacter globiformis
Expression system: Escherichia coli
UniProt:
  • Canonical: P46881 (Residues: 9-629; Coverage: 97%)
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand CU 1 x CU
Ligand NA 1 x NA
Ligand DOD 960 x DOD
2 modified residues:
Ligand ASA 1 x ASA
Ligand TPQ 1 x TPQ

Experiments and Validation Details

Experimental Method: Neutron Diffraction, X-ray diffraction
X-ray source: J-PARC MLF BEAMLINE BL-03, PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2
Unit cell:
a: 157.206Å b: 61.979Å c: 92.447Å
α: 90° β: 112.06° γ: 90°
R-values:
R R work R free
0.157 0.155 0.189
Expression system: Escherichia coli

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Citations

2 citation in other articles

Cavitation Damage Prediction in Mercury Target for Pulsed Spallation Neutron Source Using Monte Carlo Simulation.
Wakui et al. (2023)
1 more

1 mention without citation

Re-evaluation of protein neutron crystallography with and without X-ray/neutron joint refinement.
Murakawa et al. (2022)

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