› 7wnu

X-ray diffraction
3.2Å resolution

Mycobacterium tuberculosis Rnase J complex with 7nt RNA

Released:
DOI: 10.2210/pdb7wnu/pdb
PDB entry 7wnu coloured by chain, front view.
PDB entry 7wnu coloured by chain, side view.
PDB entry 7wnu coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into RNase J that plays an essential role in Mycobacterium tuberculosis RNA metabolism
Bao L, Hu J, Zhan B, Chi M, Li Z, Wang S, Shan C, Zhao Z, Guo Y, Ding X, Ji C, Tao S, Ni T, Zhang X, Zhao G, Li J
Nat Commun 14 2280- (2023)

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161535 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Ribonuclease J Chains: A, B
Molecule details ›
Chains: A, B
Length: 558 amino acids
Theoretical weight: 59.6 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGZ9 (Residues: 1-558; Coverage: 100%)
Gene names: Rv2752c, rnj
Sequence domains:
RNA (5'-R(P*AP*AP*AP*AP*AP*AP*A)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 7 nucleotides
Theoretical weight: 2.26 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2221
Unit cell:
a: 92.06Å b: 145.38Å c: 192.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.185 0.241
Expression systems:
  • Escherichia coli
  • Not provided

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