› 7wsi

Electron Microscopy
3.32Å resolution

Cryo-EM structure of human NTCP (wild-type) complexed with YN69202Fab

Released:
DOI: 10.2210/pdb7wsi/pdb
PDB entry 7wsi coloured by chain, front view.
PDB entry 7wsi coloured by chain, side view.
PDB entry 7wsi coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the bile acid transporter and HBV receptor NTCP.
Asami J, Kimura KT, Fujita-Fujiharu Y, Ishida H, Zhang Z, Nomura Y, Liu K, Uemura T, Sato Y, Ono M, Yamamoto M, Noda T, Shigematsu H, Drew D, Iwata S, Shimizu T, Nomura N, Ohto U
Nature 606 1021-1026 (2022)
PMID: 35580629
Related structures: EMD-32759

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212027 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Hepatic sodium/bile acid cotransporter Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 38.2 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q14973 (Residues: 1-319; Coverage: 91%)
Gene names: GIG29, NTCP, SLC10A1
Sequence domains: Sodium Bile acid symporter family
Fab heavy chain from antibody IgG clone number YN69202 Chain: H
Molecule details ›
Chain: H
Length: 249 amino acids
Theoretical weight: 26.57 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
Fab light chain from antibody IgG clone number YN69202 Chain: L
Molecule details ›
Chain: L
Length: 219 amino acids
Theoretical weight: 24.32 KDa
Source organism: Mus musculus
Expression system: Spodoptera littoralis

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.32Å
Relevant EMDB volumes: EMD-32759
Expression systems:
  • Spodoptera frugiperda
  • Spodoptera littoralis

Quick links

Citations

7 review citations

Advances in Immunotherapy for Hepatitis B.
Wang et al. (2022)
6 more

3 mentions without citation

Role of the Sodium-Dependent Organic Anion Transporter (SOAT/SLC10A6) in Physiology and Pathophysiology.
Wannowius et al. (2023)
2 more