7wtm

Electron Microscopy
3.5Å resolution

Cryo-EM structure of a yeast pre-40S ribosomal subunit - State Dis-E

Released:

Function and Biology Details

Reaction catalysed:
4 S-adenosyl-L-methionine + adenine(1779)/adenine(1780) in 18S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1779)/N(6)-dimethyladenine(1780) in 18S rRNA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero heptadecamer (preferred)
PDBe Complex ID:
PDB-CPX-216237 (preferred)
Entry contents:
16 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (17 distinct):
Small ribosomal subunit protein eS1A Chain: SB
Molecule details ›
Chain: SB
Length: 255 amino acids
Theoretical weight: 28.8 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P33442 (Residues: 1-255; Coverage: 100%)
Gene names: L9753.9, PLC1, RPS10A, RPS1A, YLR441C
Sequence domains: Ribosomal S3Ae family
Small ribosomal subunit protein eS4A Chain: SE
Molecule details ›
Chain: SE
Length: 261 amino acids
Theoretical weight: 29.47 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX35 (Residues: 1-261; Coverage: 100%)
Gene names: J2186, RPS4A, RPS7B, YJR145C
Sequence domains:
Small ribosomal subunit protein eS6A Chain: SG
Small ribosomal subunit protein eS7A Chain: SH
Small ribosomal subunit protein eS8A Chain: SI
Small ribosomal subunit protein uS4A Chain: SJ
Small ribosomal subunit protein uS17A Chain: SL
Small ribosomal subunit protein uS15 Chain: SN
Small ribosomal subunit protein uS11A Chain: SO
Small ribosomal subunit protein uS8A Chain: SW
Small ribosomal subunit protein uS12A Chain: SX
Small ribosomal subunit protein eS24A Chain: SY
Small ribosomal subunit protein eS27A Chain: Sb
Small ribosomal subunit protein eS30A Chain: Se
Pre-rRNA-processing protein PNO1 Chain: CA
Dimethyladenosine transferase Chain: JL
18S Ribosomal RNA Chain: C2

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-32791