› 7wu2

Electron Microscopy
2.8Å resolution

Cryo-EM structure of the adhesion GPCR ADGRD1 in complex with miniGs

Released:
DOI: 10.2210/pdb7wu2/pdb
PDB entry 7wu2 coloured by chain, front view.
PDB entry 7wu2 coloured by chain, side view.
PDB entry 7wu2 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of tethered agonism of the adhesion GPCRs ADGRD1 and ADGRF1.
Qu X, Qiu N, Wang M, Zhang B, Du J, Zhong Z, Xu W, Chu X, Ma L, Yi C, Han S, Shui W, Zhao Q, Wu B
OpenAccess logo Nature 604 779-785 (2022)
PMID: 35418679
Related structures: EMD-32817

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-211343 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 243 amino acids
Theoretical weight: 28.39 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 204-394; Coverage: 46%)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 351 amino acids
Theoretical weight: 38.74 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody Nb35 Chain: N
Molecule details ›
Chain: N
Length: 162 amino acids
Theoretical weight: 17.54 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Adhesion G-protein coupled receptor D1 Chain: R
Molecule details ›
Chain: R
Length: 348 amino acids
Theoretical weight: 38.17 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q6QNK2 (Residues: 545-827; Coverage: 33%)
Gene names: ADGRD1, GPR133, PGR25
Sequence domains: 7 transmembrane receptor (Secretin family)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-32817
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

8 review citations

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)
7 more

1 mention without citation

Regulation of pulmonary surfactant by the adhesion GPCR GPR116/ADGRF5 requires a tethered agonist-mediated activation mechanism.
Bridges et al. (2022)