› 7wu5

Electron Microscopy
3Å resolution

Cryo-EM structure of the adhesion GPCR ADGRF1(H565A/T567A) in complex with miniGi

Released:
DOI: 10.2210/pdb7wu5/pdb
PDB entry 7wu5 coloured by chain, front view.
PDB entry 7wu5 coloured by chain, side view.
PDB entry 7wu5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of tethered agonism of the adhesion GPCRs ADGRD1 and ADGRF1.
Qu X, Qiu N, Wang M, Zhang B, Du J, Zhong Z, Xu W, Chu X, Ma L, Yi C, Han S, Shui W, Zhao Q, Wu B
Nature 604 779-785 (2022)
PMID: 35418679
Related structures: EMD-32820

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-157882 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 241 amino acids
Theoretical weight: 27.56 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 4-57, 181-354; Coverage: 62%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 351 amino acids
Theoretical weight: 38.74 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Adhesion G-protein coupled receptor F1 Chain: R
Molecule details ›
Chain: R
Length: 674 amino acids
Theoretical weight: 74.51 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q5T601 (Residues: 251-860; Coverage: 69%)
Gene names: ADGRF1, GPR110, PGR19
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand CLR 1 x CLR
Ligand K6G 1 x K6G
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-32820
Expression system: Spodoptera frugiperda

Quick links

Citations

8 review citations

New insights into GPCR coupling and dimerisation from cryo-EM structures.
Gusach et al. (2023)
7 more

1 mention without citation

Regulation of pulmonary surfactant by the adhesion GPCR GPR116/ADGRF5 requires a tethered agonist-mediated activation mechanism.
Bridges et al. (2022)