› 7wui

Electron Microscopy
3.1Å resolution

Tethered peptide activation mechanism of adhesion GPCRs ADGRG2 and ADGRG4

Released:
DOI: 10.2210/pdb7wui/pdb
PDB entry 7wui coloured by chain, front view.
PDB entry 7wui coloured by chain, side view.
PDB entry 7wui coloured by chain, top view.
Source organisms:
Primary publication:
Tethered peptide activation mechanism of the adhesion GPCRs ADGRG2 and ADGRG4.
Xiao P, Guo S, Wen X, He QT, Lin H, Huang SM, Gou L, Zhang C, Yang Z, Zhong YN, Yang CC, Li Y, Gong Z, Tao XN, Yang ZS, Lu Y, Li SL, He JY, Wang C, Zhang L, Kong L, Sun JP, Yu X
Nature 604 771-778 (2022)
PMID: 35418677
Related structures: EMD-32836

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-211338 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 361 amino acids
Theoretical weight: 41.88 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: P63096 (Residues: 1-19, 61-181, 229-242; Coverage: 41%)
  • Canonical: P63092 (Residues: 27-67, 205-251, 266-394; Coverage: 55%)
Gene names: GNAI1, GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 358 amino acids
Theoretical weight: 39.49 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 58 amino acids
Theoretical weight: 6.38 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: P59768 (Residues: 5-62; Coverage: 82%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody-35 Chain: N
Molecule details ›
Chain: N
Length: 128 amino acids
Theoretical weight: 13.89 KDa
Source organism: Lama glama
Expression system: Escherichia phage EcSzw-2
Adhesion G-protein coupled receptor G2 Chain: R
Molecule details ›
Chain: R
Length: 1149 amino acids
Theoretical weight: 126.85 KDa
Source organism: Mus musculus
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: Q8CJ12 (Residues: 38-891; Coverage: 88%)
  • Best match: Q8CJ12-5 (Residues: 40-864)
Gene names: Adgrg2, Gpr64, Me6
Sequence domains:
scFv16 Chain: S
Molecule details ›
Chain: S
Length: 250 amino acids
Theoretical weight: 26.61 KDa
Source organism: synthetic construct
Expression system: Baculovirus expression vector pFastBac1-HM
Adhesion G-protein coupled receptor G2 Chain: L
Molecule details ›
Chain: L
Length: 15 amino acids
Theoretical weight: 1.65 KDa
Source organism: synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: Q8CJ12 (Residues: 600-613; Coverage: 1%)
Gene names: Adgrg2, Gpr64, Me6

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 4PH 1 x 4PH

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-32836
Expression systems:
  • Baculovirus expression vector pFastBac1-HM
  • Escherichia phage EcSzw-2
  • Not provided

Quick links

Citations

7 review citations

Adhesion G protein-coupled receptor gluing action guides tissue development and disease.
Sreepada et al. (2022)
6 more

1 mention without citation

Mechanistic Insights into Peptide Binding and Deactivation of an Adhesion G Protein-Coupled Receptor.
Adediwura et al. (2023)