› 7wvg

X-ray diffraction
2.6Å resolution

Crystal structure of H18 complexed with SIA28

Released:
DOI: 10.2210/pdb7wvg/pdb
PDB entry 7wvg coloured by chain, front view.
PDB entry 7wvg coloured by chain, side view.
PDB entry 7wvg coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for a human broadly neutralizing influenza A hemagglutinin stem-specific antibody including H17/18 subtypes.
Chen Y, Wang F, Yin L, Jiang H, Lu X, Bi Y, Zhang W, Shi Y, Burioni R, Tong Z, Song H, Qi J, Gao GF
OpenAccess logo Nat Commun 13 7603 (2022)
PMID: 36494358

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-224595 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (7 distinct):
Hemagglutinin Chain: A
Molecule details ›
Chain: A
Length: 322 amino acids
Theoretical weight: 36.02 KDa
Source organism: Influenza A virus
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: A0A1W5II89 (Residues: 15-336; Coverage: 57%)
Gene name: HA
Sequence domains: Haemagglutinin
Hemagglutinin HA2 chain Chain: B
Molecule details ›
Chain: B
Length: 170 amino acids
Theoretical weight: 19.59 KDa
Source organism: Influenza A virus
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: A0A1W5II89 (Residues: 344-513; Coverage: 30%)
Gene name: HA
Sequence domains: Haemagglutinin
Heavy chain of SIA28 Chain: C
Molecule details ›
Chain: C
Length: 124 amino acids
Theoretical weight: 13.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Light chain of SIA28 Chain: D
Molecule details ›
Chain: D
Length: 107 amino acids
Theoretical weight: 11.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL02U1
Spacegroup: P63
Unit cell:
a: 140.427Å b: 140.427Å c: 195.734Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.212 0.224
Expression systems:
  • Baculovirus expression vector pFastBac1-HM
  • Escherichia coli

Quick links

Citations

1 citations in other articles

Defining and Studying B Cell Receptor and TCR Interactions.
Rappazzo et al. (2023)