› 7wwu

Electron Microscopy
3.5Å resolution

ICP1 Csy complex

Released:
DOI: 10.2210/pdb7wwu/pdb
PDB entry 7wwu coloured by chain, front view.
PDB entry 7wwu coloured by chain, side view.
PDB entry 7wwu coloured by chain, top view.
Source organism: Vibrio phage ICP1_2011_A
Primary publication:
Mechanistic insights into DNA binding and cleavage by a compact type I-F CRISPR-Cas system in bacteriophage.
Zhang M, Peng R, Peng Q, Liu S, Li Z, Zhang Y, Song H, Yang J, Xing X, Wang P, Qi J, Gao GF
OpenAccess logo Proc Natl Acad Sci U S A 120 e2215098120 (2023)
PMID: 37094126
Related structures: EMD-32873

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-216226 (preferred)
Entry contents:
4 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
Csy1 Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 22.84 KDa
Source organism: Vibrio phage ICP1_2011_A
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: M1R2X3 (Residues: 1-179; Coverage: 100%)
Gene names: ICP12011A_088, csy1
Csy2 Chain: B
Molecule details ›
Chain: B
Length: 269 amino acids
Theoretical weight: 29.83 KDa
Source organism: Vibrio phage ICP1_2011_A
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: M1QWL5 (Residues: 1-248; Coverage: 100%)
Gene names: ICP12011A_087, csy2
Sequence domains: CRISPR-associated protein (Cas_Csy2)
Csy3 Chains: C, D, E, F, G, H
Molecule details ›
Chains: C, D, E, F, G, H
Length: 327 amino acids
Theoretical weight: 35.83 KDa
Source organism: Vibrio phage ICP1_2011_A
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: M1Q7R8 (Residues: 1-306; Coverage: 100%)
Gene names: ICP12011A_086, csy3
Sequence domains: CRISPR-associated protein (Cas_Csy3)
Csy4 Chain: I
Molecule details ›
Chain: I
Length: 189 amino acids
Theoretical weight: 21.17 KDa
Source organism: Vibrio phage ICP1_2011_A
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: M1R9H3 (Residues: 1-168; Coverage: 100%)
Gene names: ICP12011A_085, csy4
Sequence domains: CRISPR-associated protein (Cas_Csy4)
guide-RNA Chain: M
Molecule details ›
Chain: M
Length: 61 nucleotides
Theoretical weight: 19.35 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-32873
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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