› 7x0p

X-ray diffraction
1.5Å resolution

Crystal structure of sugar binding protein CbpD from Clostridium thermocellum

Released:
DOI: 10.2210/pdb7x0p/pdb
PDB entry 7x0p coloured by chain, front view.
PDB entry 7x0p coloured by chain, side view.
PDB entry 7x0p coloured by chain, top view.
Source organism: Acetivibrio thermocellus
Primary publication:
Deciphering Cellodextrin and Glucose Uptake in Clostridium thermocellum.
Yan F, Dong S, Liu YJ, Yao X, Chen C, Xiao Y, Bayer EA, Shoham Y, You C, Cui Q, Feng Y
mBio 13 e0147622 (2022)
PMID: 36069444

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107070 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic binding protein domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 37.5 KDa
Source organism: Acetivibrio thermocellus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A3DI69 (Residues: 19-372; Coverage: 100%)
Gene name: Cthe_2446
Sequence domains: Periplasmic binding protein domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: C2
Unit cell:
a: 69.91Å b: 63.17Å c: 69.7Å
α: 90° β: 106.2° γ: 90°
R-values:
R R work R free
0.171 0.17 0.2
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Comparative genomics reveals cellobiose hydrolysis mechanism of Ruminiclostridium thermocellum M3, a cellulosic saccharification bacterium.
Tao et al. (2022)
1 more