› 7x1y

Electron Microscopy
3.3Å resolution

Structure of the phosphorylation-site double mutant S431A/T432A of the KaiC circadian clock protein

Released:
DOI: 10.2210/pdb7x1y/pdb
PDB entry 7x1y coloured by chain, front view.
PDB entry 7x1y coloured by chain, side view.
PDB entry 7x1y coloured by chain, top view.
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Primary publication:
Determining subunit-subunit interaction from statistics of cryo-EM images: observation of nearest-neighbor coupling in a circadian clock protein complex
Han X, Zhang D, Hong L, Yu D, Wu Z, Yang T, Rust M, Tu Y, Ouyang Q
Nat Commun 14 5907- (2023)
Related structures: EMD-32952

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Circadian clock oscillator protein KaiC Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 471 amino acids
Theoretical weight: 52.76 KDa
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q79PF4 (Residues: 14-484; Coverage: 91%)
Gene names: Synpcc7942_1216, kaiC, see0011
Sequence domains: KaiC

Ligands and Environments

2 bound ligands:
Ligand ATP 12 x ATP
Ligand MG 12 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-32952
Expression system: Escherichia coli BL21(DE3)

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