› 7x2o

Electron Microscopy
3.15Å resolution

Cryo-EM structure of Coxsackievirus B1 mature virion in complex with nAb 2E6 (CVB1-M:2E6)

Released:
DOI: 10.2210/pdb7x2o/pdb
PDB entry 7x2o coloured by chain, front view.
PDB entry 7x2o coloured by chain, side view.
PDB entry 7x2o coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the synergistic neutralization of coxsackievirus B1 by a triple-antibody cocktail.
Zheng Q, Zhu R, Yin Z, Xu L, Sun H, Yu H, Wu Y, Jiang Y, Huang Q, Huang Y, Zhang D, Liu L, Yang H, He M, Zhou Z, Jiang Y, Chen Z, Zhao H, Que Y, Kong Z, Zhou L, Li T, Zhang J, Luo W, Gu Y, Cheng T, Li S, Xia N
Cell Host Microbe 30 1279-1294.e6 (2022)
PMID: 36002016
Related structures: EMD-32969

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 360-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-224685 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Virion protein 1 Chain: A
Molecule details ›
Chain: A
Length: 278 amino acids
Theoretical weight: 31.21 KDa
Source organism: Coxsackievirus B1
UniProt:
  • Canonical: A0A7T7KAA0 (Residues: 571-848; Coverage: 33%)
Sequence domains: picornavirus capsid protein
VP2 Chain: B
Molecule details ›
Chain: B
Length: 263 amino acids
Theoretical weight: 29.12 KDa
Source organism: Coxsackievirus B1
UniProt:
  • Canonical: A0A7T7KAA0 (Residues: 70-332; Coverage: 31%)
Sequence domains: picornavirus capsid protein
VP3 Chain: C
Molecule details ›
Chain: C
Length: 238 amino acids
Theoretical weight: 26.33 KDa
Source organism: Coxsackievirus B1
UniProt:
  • Canonical: A0A7T7KAA0 (Residues: 333-570; Coverage: 28%)
Sequence domains: picornavirus capsid protein
Capsid protein VP4 Chain: D
Molecule details ›
Chain: D
Length: 69 amino acids
Theoretical weight: 7.48 KDa
Source organism: Coxsackievirus B1
UniProt: Sequence domains: Picornavirus coat protein (VP4)
2E6 light chain Chain: L
Molecule details ›
Chain: L
Length: 107 amino acids
Theoretical weight: 11.72 KDa
Source organism: Mus musculus
2E6 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 119 amino acids
Theoretical weight: 13.09 KDa
Source organism: Mus musculus

Ligands and Environments

1 bound ligand:
Ligand PLM 1 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.15Å
Relevant EMDB volumes: EMD-32969

Quick links

Citations

1 citations in other articles

A counterintuitive antibody cocktail disrupts coxsackievirus.
Zost et al. (2022)