› 7x35

Electron Microscopy
3.19Å resolution

Cryo-EM structure of Coxsackievirus B1 A-particle in complex with nAb 8A10 (CVB1-A:8A10)

Released:
DOI: 10.2210/pdb7x35/pdb
PDB entry 7x35 coloured by chain, front view.
PDB entry 7x35 coloured by chain, side view.
PDB entry 7x35 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the synergistic neutralization of coxsackievirus B1 by a triple-antibody cocktail.
Zheng Q, Zhu R, Yin Z, Xu L, Sun H, Yu H, Wu Y, Jiang Y, Huang Q, Huang Y, Zhang D, Liu L, Yang H, He M, Zhou Z, Jiang Y, Chen Z, Zhao H, Que Y, Kong Z, Zhou L, Li T, Zhang J, Luo W, Gu Y, Cheng T, Li S, Xia N
Cell Host Microbe 30 1279-1294.e6 (2022)
PMID: 36002016
Related structures: EMD-32979

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 300-mer (preferred)
PDBe Complex ID:
PDB-CPX-251888 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Virion protein 1 Chain: A
Molecule details ›
Chain: A
Length: 278 amino acids
Theoretical weight: 31.21 KDa
Source organism: Coxsackievirus B1
UniProt:
  • Canonical: A0A7T7KAA0 (Residues: 571-848; Coverage: 33%)
Sequence domains: picornavirus capsid protein
VP2 Chain: B
Molecule details ›
Chain: B
Length: 263 amino acids
Theoretical weight: 29.12 KDa
Source organism: Coxsackievirus B1
UniProt:
  • Canonical: A0A2S0RQC2 (Residues: 70-332; Coverage: 12%)
Sequence domains: picornavirus capsid protein
VP3 Chain: C
Molecule details ›
Chain: C
Length: 238 amino acids
Theoretical weight: 26.33 KDa
Source organism: Coxsackievirus B1
UniProt:
  • Canonical: L7UV52 (Residues: 333-570; Coverage: 11%)
Sequence domains: picornavirus capsid protein
8A10 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 118 amino acids
Theoretical weight: 13.32 KDa
Source organism: Mus musculus
8A10 light chain Chain: L
Molecule details ›
Chain: L
Length: 108 amino acids
Theoretical weight: 11.95 KDa
Source organism: Mus musculus

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.19Å
Relevant EMDB volumes: EMD-32979

Quick links

Citations

2 citation in other articles

A counterintuitive antibody cocktail disrupts coxsackievirus.
Zost et al. (2022)
1 more