› 7x42

Electron Microscopy
3.88Å resolution

Cryo-EM structure of Coxsackievirus B1 pre-A-particle in complex with nAb 8A10 (classified from CVB1 mature virion in complex with 8A10 and 2E6)

Released:
DOI: 10.2210/pdb7x42/pdb
PDB entry 7x42 coloured by chain, front view.
PDB entry 7x42 coloured by chain, side view.
PDB entry 7x42 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the synergistic neutralization of coxsackievirus B1 by a triple-antibody cocktail.
Zheng Q, Zhu R, Yin Z, Xu L, Sun H, Yu H, Wu Y, Jiang Y, Huang Q, Huang Y, Zhang D, Liu L, Yang H, He M, Zhou Z, Jiang Y, Chen Z, Zhao H, Que Y, Kong Z, Zhou L, Li T, Zhang J, Luo W, Gu Y, Cheng T, Li S, Xia N
Cell Host Microbe 30 1279-1294.e6 (2022)
PMID: 36002016
Related structures: EMD-32999

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 360-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-279067 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
8A10 light chain Chain: L
Molecule details ›
Chain: L
Length: 108 amino acids
Theoretical weight: 11.95 KDa
Source organism: Mus musculus
8A10 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 118 amino acids
Theoretical weight: 13.32 KDa
Source organism: Mus musculus
Virion protein 1 Chain: A
Molecule details ›
Chain: A
Length: 278 amino acids
Theoretical weight: 31.21 KDa
Source organism: Coxsackievirus B1
UniProt:
  • Canonical: A0A7T7KAA0 (Residues: 571-848; Coverage: 33%)
Sequence domains: picornavirus capsid protein
Capsid protein VP0 Chain: B
Molecule details ›
Chain: B
Length: 263 amino acids
Theoretical weight: 29.12 KDa
Source organism: Coxsackievirus B1
UniProt:
  • Canonical: A0A7T7KAA0 (Residues: 70-332; Coverage: 31%)
Sequence domains: picornavirus capsid protein
VP3 Chain: C
Molecule details ›
Chain: C
Length: 238 amino acids
Theoretical weight: 26.33 KDa
Source organism: Coxsackievirus B1
UniProt:
  • Canonical: A0A7T7KAA0 (Residues: 333-570; Coverage: 28%)
Sequence domains: picornavirus capsid protein
Capsid protein VP4 Chain: D
Molecule details ›
Chain: D
Length: 69 amino acids
Theoretical weight: 7.48 KDa
Source organism: Coxsackievirus B1
UniProt: Sequence domains: Picornavirus coat protein (VP4)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.88Å
Relevant EMDB volumes: EMD-32999

Quick links

Citations

2 citation in other articles

A counterintuitive antibody cocktail disrupts coxsackievirus.
Zost et al. (2022)
1 more

1 mention without citation

Motions around conserved helical weak spots facilitate GPCR activation.
Bibbe et al. (2021)