› 7x4l

X-ray diffraction
2.59Å resolution

Crystal structure of Bacteroides thetaiotaomicron glutamate decarboxylase mutant Y303F-PLP complex

Released:
DOI: 10.2210/pdb7x4l/pdb
PDB entry 7x4l coloured by chain, front view.
PDB entry 7x4l coloured by chain, side view.
PDB entry 7x4l coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Primary publication:
Coordinated regulation of Bacteroides thetaiotaomicron glutamate decarboxylase activity by multiple elements under different pH.
Liu S, Wen B, Du G, Wang Y, Ma X, Yu H, Zhang J, Fan S, Zhou H, Xin F
Food Chem 403 134436 (2023)
PMID: 36358099

Function and Biology Details

Reaction catalysed: 
L-glutamate = 4-aminobutanoate + CO(2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-199944 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate decarboxylase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 481 amino acids
Theoretical weight: 54.9 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8A4M9 (Residues: 1-481; Coverage: 100%)
Gene name: BT_2570
Sequence domains: Pyridoxal-dependent decarboxylase conserved domain

Ligands and Environments


Cofactor: Ligand PLP 6 x PLP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 155.449Å b: 176.383Å c: 176.797Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.203 0.214
Expression system: Escherichia coli

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Citations

1 review citation

The Diagnostic Potential of the Human Blood Microbiome: Are We Dreaming or Awake?
Sciarra et al. (2023)