› 7x4t

X-ray diffraction
2.2Å resolution

LpCdnE UMPNPP Mg complex

Released:
DOI: 10.2210/pdb7x4t/pdb
PDB entry 7x4t coloured by chain, front view.
PDB entry 7x4t coloured by chain, side view.
PDB entry 7x4t coloured by chain, top view.
Source organism: Legionella pneumophila
Primary publication:
Crystal structure and functional implications of cyclic di-pyrimidine-synthesizing cGAS/DncV-like nucleotidyltransferases.
Yang CS, Ko TP, Chen CJ, Hou MH, Wang YC, Chen Y
OpenAccess logo Nat Commun 14 5078 (2023)
PMID: 37604815

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic dipyrimidine nucleotide synthase CdnE Chains: A, B
Molecule details ›
Chains: A, B
Length: 303 amino acids
Theoretical weight: 34.96 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0DSP3 (Residues: 1-295; Coverage: 100%)
Gene name: cdnE02
Sequence domains: Nucleotidyltransferase domain

Ligands and Environments

4 bound ligands:
Ligand 2KH 4 x 2KH
Ligand MG 5 x MG
Ligand SO4 2 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE TPS 05A
Spacegroup: P21212
Unit cell:
a: 157.495Å b: 72.91Å c: 66.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.159 0.197
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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