› 7x5b

X-ray diffraction
2.16Å resolution

Crystal structure of RuvB

Released:
DOI: 10.2210/pdb7x5b/pdb
PDB entry 7x5b coloured by chain, front view.
PDB entry 7x5b coloured by chain, side view.
PDB entry 7x5b coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Cryo-EM structure of the RuvAB-Holliday junction intermediate complex from Pseudomonas aeruginosa.
Zhang X, Zhou Z, Dai L, Chao Y, Liu Z, Huang M, Qu Q, Lin Z
OpenAccess logo Front Plant Sci 14 1139106 (2023)
PMID: 37025142

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175937 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Holliday junction branch migration complex subunit RuvB Chain: A
Molecule details ›
Chain: A
Length: 352 amino acids
Theoretical weight: 38.97 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q51426 (Residues: 1-352; Coverage: 100%)
Gene names: PA0967, ruvB
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P65
Unit cell:
a: 85.677Å b: 85.677Å c: 76.973Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.173 0.217
Expression system: Escherichia coli BL21(DE3)

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Citations

1 mention without citation

Cryo-EM structure of the RuvAB-Holliday junction intermediate complex from Pseudomonas aeruginosa.
Zhang et al. (2023)