› 7x6i

Electron Microscopy
3.93Å resolution

Cryo-EM structure of the human TRPC5 ion channel in complex with G alpha i3 subunits, class1

Released:
DOI: 10.2210/pdb7x6i/pdb
PDB entry 7x6i coloured by chain, front view.
PDB entry 7x6i coloured by chain, side view.
PDB entry 7x6i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular architecture of the Gαi-bound TRPC5 ion channel.
Won J, Kim J, Jeong H, Kim J, Feng S, Jeong B, Kwak M, Ko J, Im W, So I, Lee HH
OpenAccess logo Nat Commun 14 2550 (2023)
PMID: 37137991
Related structures: EMD-33022

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-216274 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Short transient receptor potential channel 5 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 773 amino acids
Theoretical weight: 89.95 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9UL62 (Residues: 1-765; Coverage: 79%)
Gene names: TRP5, TRPC5
Sequence domains:
Guanine nucleotide-binding protein G(i) subunit alpha-3 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 360 amino acids
Theoretical weight: 41.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P08754 (Residues: 1-119, 120-354; Coverage: 100%)
Gene name: GNAI3
Sequence domains: G-protein alpha subunit

Ligands and Environments

6 bound ligands:
Ligand PTY 4 x PTY
Ligand Y01 4 x Y01
Ligand ZN 4 x ZN
Ligand CA 4 x CA
Ligand YZY 4 x YZY
Ligand GTP 4 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.93Å
Relevant EMDB volumes: EMD-33022
Expression systems:
  • Homo sapiens
  • Escherichia coli K-12

Quick links

Citations

8 citation in other articles

Dissecting the molecular basis for the modulation of neurotransmitter GPCR signaling by GINIP.
Luebbers et al. (2024)
7 more

2 mentions without citation

Direct modulation of TRPC ion channels by Gα proteins.
Kang et al. (2024)
1 more