› 7x7t

Electron Microscopy
3.48Å resolution

Cryo-EM structure of SARS-CoV-2 spike protein in complex with three nAbs X01, X10 and X17

Released:
DOI: 10.2210/pdb7x7t/pdb
PDB entry 7x7t coloured by chain, front view.
PDB entry 7x7t coloured by chain, side view.
PDB entry 7x7t coloured by chain, top view.
Source organisms:
Primary publication:
The neutralizing breadth of antibodies targeting diverse conserved epitopes between SARS-CoV and SARS-CoV-2.
Xiong H, Sun H, Wang S, Yuan L, Liu L, Zhu Y, Zhang J, Huang Y, Qi R, Jiang Y, Ma J, Zhou M, Ma Y, Fu R, Yan S, Yue M, Wu Y, Wei M, Wang Y, Li T, Wang Y, Zheng Z, Yu H, Cheng T, Li S, Yuan Q, Zhang J, Guan Y, Zheng Q, Zhang T, Xia N
OpenAccess logo Proc Natl Acad Sci U S A 119 e2204256119 (2022)
PMID: 35972965
Related structures: EMD-33047

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-225246 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Spike protein S1 Chain: G
Molecule details ›
Chain: G
Length: 204 amino acids
Theoretical weight: 22.93 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DTC2 (Residues: 324-527; Coverage: 16%)
Gene names: 2, S
Sequence domains: Betacoronavirus spike glycoprotein S1, receptor binding
X10 light chain Chain: L
Molecule details ›
Chain: L
Length: 111 amino acids
Theoretical weight: 12.1 KDa
Source organism: Mus musculus
X10 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 121 amino acids
Theoretical weight: 13.4 KDa
Source organism: Mus musculus
X17 light chain Chain: B
Molecule details ›
Chain: B
Length: 107 amino acids
Theoretical weight: 11.77 KDa
Source organism: Mus musculus
X17 heavy chain Chain: A
Molecule details ›
Chain: A
Length: 119 amino acids
Theoretical weight: 13.15 KDa
Source organism: Mus musculus
X01 light chain Chain: D
Molecule details ›
Chain: D
Length: 107 amino acids
Theoretical weight: 11.78 KDa
Source organism: Mus musculus
X01 heavy chain Chain: C
Molecule details ›
Chain: C
Length: 119 amino acids
Theoretical weight: 13.06 KDa
Source organism: Mus musculus

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.48Å
Relevant EMDB volumes: EMD-33047
Expression system: Homo sapiens

Quick links

Citations

10 citation in other articles

The neutralizing breadth of antibodies targeting diverse conserved epitopes between SARS-CoV and SARS-CoV-2.
Xiong et al. (2022)
9 more

1 mention without citation

Targeting the Spike Receptor Binding Domain Class V Cryptic Epitope by an Antibody with Pan-Sarbecovirus Activity.
Jensen et al. (2023)