› 7x8t

X-ray diffraction
2.51Å resolution

Frizzled 10 CRD in complex with hB9L9.3 Fab

Released:
DOI: 10.2210/pdb7x8t/pdb
PDB entry 7x8t coloured by chain, front view.
PDB entry 7x8t coloured by chain, side view.
PDB entry 7x8t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An epitope-directed selection strategy facilitating the identification of Frizzled receptor selective antibodies.
Ge Q, Teng M, Li X, Guo Q, Tao Y
Structure 31 33-43.e5 (2023)
PMID: 36513066

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-226381 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Frizzled-10 Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 129 amino acids
Theoretical weight: 14.9 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9ULW2 (Residues: 24-152; Coverage: 23%)
Gene name: FZD10
Sequence domains: Fz domain
Antibody hB9L9.3 Fab, Light chain Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 212 amino acids
Theoretical weight: 22.38 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Antibody hB9L9.3 Fab, Heavy chain Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 237 amino acids
Theoretical weight: 25.02 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P1
Unit cell:
a: 75.456Å b: 100.794Å c: 99.149Å
α: 110.074° β: 89.935° γ: 113.793°
R-values:
R R work R free
0.178 0.176 0.215
Expression system: Homo sapiens

Quick links

Citations

2 citation in other articles

A method for structure determination of GPCRs in various states.
Guo et al. (2024)
1 more