› 7xd5

Electron Microscopy
2.84Å resolution

The Tet-S2 state of wild-type Tetrahymena group I intron with 30nt 3'/5'-exon

Released:
DOI: 10.2210/pdb7xd5/pdb
PDB entry 7xd5 coloured by chain, front view.
PDB entry 7xd5 coloured by chain, side view.
PDB entry 7xd5 coloured by chain, top view.
Source organism: Tetrahymena thermophila
Primary publication:
Cryo-EM reveals dynamics of Tetrahymena group I intron self-splicing
Luo B, Zhang C, Ling X, Mukherjee S, Jia G, Xie J, Jia X, Liu L, Baulin EF, Luo Y, Jiang L, Dong H, Wei X, Bujnicki JM, Su Z
Nat Catal (2023)
Related structures: EMD-33136

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-199964 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
The Tet-S2 state molecule of co-transcriptional folded wild-type Tetrahymena group I intron with 30nt 3'/5'-exon (intron and 3'-exon) Chain: N
Molecule details ›
Chain: N
Length: 400 nucleotides
Theoretical weight: 129.22 KDa
Source organism: Tetrahymena thermophila
Expression system: synthetic construct
Sequence domains: Group I catalytic intron
The Tet-S2 state molecule of co-transcriptional folded wild-type Tetrahymena group I intron with 30nt 3'/5'-exon (5'-exon) Chain: A
Molecule details ›
Chain: A
Length: 6 nucleotides
Theoretical weight: 1.79 KDa
Source organism: Tetrahymena thermophila
Expression system: synthetic construct

Ligands and Environments

2 bound ligands:
Ligand SPD 1 x SPD
Ligand MG 29 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.84Å
Relevant EMDB volumes: EMD-33136
Expression system: synthetic construct

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