› 7xej

X-ray diffraction
1.74Å resolution

SufS with D-cysteine for 5 min

Released:
DOI: 10.2210/pdb7xej/pdb
PDB entry 7xej coloured by chain, front view.
PDB entry 7xej coloured by chain, side view.
PDB entry 7xej coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Entry authors: Nakamiura R, Fujishiro T

Function and Biology Details

Reaction catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128426 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine desulfurase SufS Chain: A
Molecule details ›
Chain: A
Length: 419 amino acids
Theoretical weight: 46.59 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O32164 (Residues: 1-406; Coverage: 100%)
Gene names: BSU32690, csd, sufS, yurW
Sequence domains: Aminotransferase class-V

Ligands and Environments

4 bound ligands:
Ligand 9PV 1 x 9PV
Ligand EDO 1 x EDO
Ligand PEG 7 x PEG
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P3121
Unit cell:
a: 92.9Å b: 92.9Å c: 129.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.146 0.145 0.176
Expression system: Escherichia coli BL21(DE3)

Quick links