7xg0

Electron Microscopy
2.6Å resolution

CryoEM structure of type IV-A Csf-crRNA-dsDNA ternary complex

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-222855 (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (7 distinct):
Csf1 Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 28.68 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
Csf3 Chain: B
Molecule details ›
Chain: B
Length: 220 amino acids
Theoretical weight: 24.42 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
Csf2 Chains: C, D, E, F, G
Molecule details ›
Chains: C, D, E, F, G
Length: 348 amino acids
Theoretical weight: 37.32 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
Csf5 Chain: H
Molecule details ›
Chain: H
Length: 268 amino acids
Theoretical weight: 29.15 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
NTS Chain: J
Molecule details ›
Chain: J
Length: 54 nucleotides
Theoretical weight: 16.73 KDa
TS Chain: K
Molecule details ›
Chain: K
Length: 54 nucleotides
Theoretical weight: 16.55 KDa
crRNA Chain: I
Molecule details ›
Chain: I
Length: 61 nucleotides
Theoretical weight: 19.9 KDa

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 2.6Å
Relevant EMDB volumes: EMD-33181
Expression system: Escherichia coli