› 7xg4

Electron Microscopy
3.7Å resolution

CryoEM structure of type IV-A CasDinG bound NTS-nicked Csf-crRNA-dsDNA quaternary complex in a second state

Released:
DOI: 10.2210/pdb7xg4/pdb
PDB entry 7xg4 coloured by chain, front view.
PDB entry 7xg4 coloured by chain, side view.
PDB entry 7xg4 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Type IV-A CRISPR-Csf complex: Assembly, dsDNA targeting, and CasDinG recruitment.
Cui N, Zhang JT, Liu Y, Liu Y, Liu XY, Wang C, Huang H, Jia N
Mol Cell (2023)
PMID: 37343553
Related structures: EMD-33185

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-222858 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
Csf1 Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 28.68 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
Csf3 Chain: B
Molecule details ›
Chain: B
Length: 220 amino acids
Theoretical weight: 24.42 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
Csf2 Chains: C, D, E, F, G
Csf5 Chain: H
Csf4 Chain: L
NTS Chain: J
TS Chain: K
crRNA Chain: I

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
1 modified residue:
Ligand 23G 1 x 23G

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-33185
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Helicases in R-loop Formation and Resolution.
Yang et al. (2023)
1 more