› 7xhb

Electron Microscopy
3.33Å resolution

Structure of the SecA/SecYE/proOmpA(4Y)-sfGFP complex with ADP

Released:
DOI: 10.2210/pdb7xhb/pdb
PDB entry 7xhb coloured by chain, front view.
PDB entry 7xhb coloured by chain, side view.
PDB entry 7xhb coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of SecA-mediated protein translocation.
Dong L, Yang S, Chen J, Wu X, Sun D, Song C, Li L
OpenAccess logo Proc Natl Acad Sci U S A 120 e2208070120 (2023)
PMID: 36598944
Related structures: EMD-33193

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + cellular protein(Side 1) = ADP + phosphate + cellular protein(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-107331 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Protein translocase subunit SecA Chain: A
Molecule details ›
Chain: A
Length: 778 amino acids
Theoretical weight: 88.67 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P28366 (Residues: 1-778; Coverage: 93%)
Gene names: BSU35300, div+, secA
Sequence domains:
Protein translocase subunit SecY Chain: Y
Molecule details ›
Chain: Y
Length: 430 amino acids
Theoretical weight: 47.63 KDa
Source organism: Geobacillus thermodenitrificans NG80-2
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: A4IJK8 (Residues: 1-430; Coverage: 100%)
Gene names: GTNG_0125, secY
Sequence domains: SecY
Protein translocase subunit SecE Chain: E
Molecule details ›
Chain: E
Length: 60 amino acids
Theoretical weight: 7.03 KDa
Source organism: Geobacillus thermodenitrificans NG80-2
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: A4IJH4 (Residues: 1-60; Coverage: 100%)
Gene names: GTNG_0091, secE
Sequence domains: SecE/Sec61-gamma subunits of protein translocation complex
Translocating peptide Chain: B
Molecule details ›
Chain: B
Length: 345 amino acids
Theoretical weight: 37.38 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli K-12

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.33Å
Relevant EMDB volumes: EMD-33193
Expression system: Escherichia coli K-12

Quick links

Citations

1 review citation

A unifying mechanism for protein transport through the core bacterial Sec machinery.
Allen et al. (2023)
1 other article