› 7xjf

X-ray diffraction
2.6Å resolution

Crystal structure of 6MW3211 Fab in complex with CD47

Released:
DOI: 10.2210/pdb7xjf/pdb
PDB entry 7xjf coloured by chain, front view.
PDB entry 7xjf coloured by chain, side view.
PDB entry 7xjf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Blockade of dual immune checkpoint inhibitory signals with a CD47/PD-L1 bispecific antibody for cancer treatment.
Wang R, Zhang C, Cao Y, Wang J, Jiao S, Zhang J, Wang M, Tang P, Ouyang Z, Liang W, Mao Y, Wang A, Li G, Zhang J, Wang M, Wang S, Gui X
OpenAccess logo Theranostics 13 148-160 (2023)
PMID: 36593962

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-226209 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Heavy chain of 6MW3211 Fab Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 24.47 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Light chain of 6MW3211 Fab Chain: B
Molecule details ›
Chain: B
Length: 214 amino acids
Theoretical weight: 23.43 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Leukocyte surface antigen CD47 Chain: C
Molecule details ›
Chain: C
Length: 116 amino acids
Theoretical weight: 13.09 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: Q08722 (Residues: 19-134; Coverage: 38%)
Gene names: CD47, MER6
Sequence domains: CD47 immunoglobulin-like domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
2 bound ligands:
Ligand CL 2 x CL
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NFPSS BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 60.83Å b: 100.48Å c: 163.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.231
Expression systems:
  • Homo sapiens
  • Cricetulus griseus

Quick links

Citations

1 review citation

Revolutionizing cancer immunotherapy: unleashing the potential of bispecific antibodies for targeted treatment.
Guo et al. (2023)
4 other articles

1 mention without citation

Blockade of dual immune checkpoint inhibitory signals with a CD47/PD-L1 bispecific antibody for cancer treatment.
Wang et al. (2023)