7xjg

Electron Microscopy
2.51Å resolution

Cryo-EM structure of E.coli retron-Ec86 in complex with its effector at 2.5 angstrom

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-122302 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
2 distinct RNA molecules
Macromolecules (5 distinct):
Retron Ec86 reverse transcriptase Chains: A, B
Molecule details ›
Chains: A, B
Length: 330 amino acids
Theoretical weight: 37.83 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23070 (Residues: 1-320; Coverage: 100%)
Gene names: LM2_00877, ret
Sequence domains: Reverse transcriptase (RNA-dependent DNA polymerase)
Retron Ec86 putative ribosyltransferase/DNA-binding protein Chains: C, J
Molecule details ›
Chains: C, J
Length: 307 amino acids
Theoretical weight: 35.54 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DV88 (Residues: 1-307; Coverage: 100%)
Gene name: LM2_00875
DNA (105-MER) Chains: D, G
RNA (81-MER) Chains: E, H
RNA (14-MER) Chains: F, I

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.51Å
Relevant EMDB volumes: EMD-33226
Expression system: Escherichia coli BL21(DE3)