› 7xji

Electron Microscopy
3.9Å resolution

Solabegron-activated dog beta3 adrenergic receptor

Released:
DOI: 10.2210/pdb7xji/pdb
PDB entry 7xji coloured by chain, front view.
PDB entry 7xji coloured by chain, side view.
PDB entry 7xji coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structures of the β3 adrenergic receptor bound to solabegron and isoproterenol.
Nureki I, Kobayashi K, Tanaka T, Demura K, Inoue A, Shihoya W, Nureki O
Biochem Biophys Res Commun 611 158-164 (2022)
PMID: 35489202
Related structures: EMD-33228

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-208013 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 44.26 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 351 amino acids
Theoretical weight: 38.74 KDa
Source organism: Rattus norvegicus
Expression system: Baculovirus expression vector pFastBac1-HM
Nanobody-35 Chain: N
Molecule details ›
Chain: N
Length: 137 amino acids
Theoretical weight: 15.02 KDa
Source organism: unidentified
Expression system: Escherichia coli
Beta-3 adrenergic receptor Chain: R
Molecule details ›
Chain: R
Length: 395 amino acids
Theoretical weight: 42.44 KDa
Source organism: Canis lupus familiaris
Expression system: Baculovirus expression vector pFastBac1-HM
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 68 amino acids
Theoretical weight: 7.55 KDa
Source organism: Bos taurus
Expression system: Baculovirus expression vector pFastBac1-HM

Ligands and Environments

1 bound ligand:
Ligand EI5 1 x EI5
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-33228
Expression systems:
  • Baculovirus expression vector pFastBac1-HM
  • Escherichia coli

Quick links

Citations

3 review citations

Pharmacological Support for the Treatment of Obesity-Present and Future.
Kosmalski et al. (2023)
2 more